Neutralization Assay Services
neutralization ASSAY services
Let Us Generate Your Neutralization Data
Neutralization assays are powerful techniques to determine the inhibitory effect of antibodies or sera on virus infectivity. When the antibodies or sera are tested in a dose-response manner, these assays determine the concentration of serum or antibody required to neutralize 50% of infection (IC50) and allow comparison between different treatments.
Our Approach
How our neutralization assays are done.
To determine the neutralizing properties of serum or antibodies, at VRS, we use an immunofluorescence-based microneutralization assay.
In this assay, a serial dilution of serum, antibody, or even a compound-of-interest is incubated with a pre-optimized concentration of virus before the complex is added to permissive cells. After fixation, infection is detected by immunostaining of a viral antigen and quantified by high-content imaging. Infection percentages are normalized to untreated-uninfected and untreated-infected controls, which are included in each plate.
A microneutralization assay is performed in 96-well plate format and, therefore, is ideal when the volume of serum is limited. Our microneutralization assay is optimized to reduce the volume of testing material required, allowing for multiple tests against different viral strains, variants, or serotypes, multiple assays, and multiple replicates/repeats for increased robustness.
The performance of all our neutralization assays is controlled across experiments using purified antibodies or sera.
Neutralization and microneutralization assays are readily available for the following live, replicating viruses:
• SARS-CoV-2 and all variants of concern
• Respiratory syncytial virus (RSV)
• Dengue virus (serotypes 1-4)
• Zika virus (African and Asian lineages)
• Human immunodeficiency virus type 1 (HIV-1)
• Vaccinia virus
• Human cytomegalovirus (HCMV, Merlin strain)
Other viruses may be available on request.
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