Real-time PCR Services

qPCR ASSAY SERVICE

Let Us Run Your qPCR

Fast, sensitive, and reliable quantitative real-time PCR (qPCR) is the gold standard for precise quantification of target nucleic acids.

At VRS, we provide qPCR services for viral detection and quantification, determination of viral copy number, drug/treatment response, gene expression analysis, relative and absolute quantification, mutation analysis, and genotyping.

Our Approach

How our qPCR assays are done.

 Our quantitative PCR services include:
•Design consultation to understand your qPCR application
•Choice of the desired chemistry (SYBR™ Green or TaqMan)
•DNA/RNA extraction from fluids, cells, and tissues
•cDNA synthesis or one-step reaction strategies
•Primer design and validation
•Design of probes and quencher probes
•Identification of suitable controls
•Use of validated viral standards for quantification
•Amplification efficiency assessed for each assay using standard curves
•Data analysis
•Full professional report (plus analyzed and raw data) 

We offer services to rapidly assay samples from cultured cells, supernatants, human preclinical tissues and animal, plant, and viral samples.

Our Viruses

Cytomegalovirus (CMV)
Dengue virus (serotypes 1-4)
Human coronavirus NL63 (HCoV-NL63)
Human coronavirus 229E (HCoV-229E)
Human immunodeficiency virus (HIV)
Influenza (A and B)
Respiratory syncytial virus (RSV)
Ross River virus
SARS-CoV-2
Semliki Forest virus
Sindbis virus
Vaccinia virus
Zika virus (African and Asian)

Amplification of other viruses available on request.

PCR Custom Assays

Samples, primers, and probes for your qPCR experiment can be provided by the customers or developed by us.

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